Desmoplakin antibody (AA 1546-1558)

Catalog No. ABIN570872

The Goat Polyclonal anti-Desmoplakin antibody has been validated for ELISA. It is suitable to detect Desmoplakin in samples from Human.

Quick Overview for Desmoplakin antibody (AA 1546-1558) (ABIN570872)

Target: Desmoplakin (DSP)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Desmoplakin antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-Desmoplakin Antibody

Binding Specificity: AA 1546-1558

Purpose: Desmoplakin (aa 1546-1558)

Sequence: SQERTVKDQD ITR

Specificity: This antibody is expected to recognize reported isoform I (NP_004406.2) only.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-SQERTVKDQDITR

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Desmoplakin

Target: Desmoplakin (DSP)

Alternative Name: DSP

Background: DSP, desmoplakin, DPI, 250 kDa paraneoplastic pemphigus antigen, desmoplakin I,

Gene ID: 1832, 109620, 306871

NCBI Accession: NP_004406

Pathways: Cell-Cell Junction Organization