LY6E antibody (Internal Region)

Catalog No. ABIN570878

The Goat Polyclonal anti-LY6E antibody is suitable to detect LY6E in samples from Mouse. It has been validated for ELISA, IF, IHC and FACS.

Quick Overview for LY6E antibody (Internal Region) (ABIN570878)

Target: LY6E (Lymphocyte Antigen 6 Complex, Locus E (LY6E))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This LY6E antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-LY6E Antibody

Binding Specificity: Internal Region

Purpose: LY6E (mouse)

Sequence: SCQEKDHYCI T

Specificity: Reported variants represent identical protein: NP_002337.1, NP_001120685.1

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: SCQEKDHYCIT

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Customer obtained staining in Mouse Placenta in a pattern consistent with in situ hybridisation results, and no staining in KO mice. This antibody has been successfully used in IHC on Mouse: M. Hughes et al, Placenta. 2013 Sep,34(9)

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membrane of NIH3T3 cells. Recommended concentration: 10μg/ml

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/m

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LY6E

Target: LY6E (Lymphocyte Antigen 6 Complex, Locus E (LY6E))

Alternative Name: LY6E

Background: LY6E, lymphocyte antigen 6 complex, locus E, RIG-E, RIGE, SCA-2, SCA2, TSA-1, retinoic acid induced gene E

Gene ID: 17069

NCBI Accession: NP_002337, NP_032555

Pathways: Hormone Transport