ILK antibody (C-Term)

Catalog No. ABIN570904

This Goat Polyclonal antibody specifically detects ILK in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for ILK antibody (C-Term) (ABIN570904)

Target: ILK (Integrin-Linked Kinase (ILK))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ILK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ILK Antibody

Binding Specificity: C-Term

Purpose: ILK

Sequence: DMIVPILEKM QDK

Specificity: All 3 varients (represented by NP_004508.1, NP_001014794.1 and NP_001014795.1) encode the same protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-DMIVPILEKMQDK

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 52 kDa band observed in Mouse Kidney lysates and in lysates of cell line NIH3T3 and 50-51 kDa in HEK293, A431, HeLa and Jurkat lysates (calculated MW of 51.4 kDa according to Human NP_004508.1 and Mouse NP_034692.2). Recommended concent

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ILK

Target: ILK (Integrin-Linked Kinase (ILK))

Alternative Name: ILK

Background: ILK, integrin-linked kinase, P59, DKFZp686F1765

Gene ID: 3611

NCBI Accession: NP_004508, NP_001014794, NP_001014795

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration