GATA3 antibody (N-Term)

Catalog No. ABIN570910

This Goat Polyclonal antibody specifically detects GATA3 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for GATA3 antibody (N-Term) (ABIN570910)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GATA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GATA3 Antibody

Binding Specificity: N-Term

Purpose: GATA3

Sequence: EVTADQPRWV SHH

Specificity: This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence EVTADQPRWVSHH-C, from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 46 kDa band observed in Human Tonsil and in Mouse Kidney lysates. (calculated MW of 47.9 kDa according to NP_002042.1 and Mouse NP_032117.1 ). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GATA3

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Alternative Name: GATA3

Background: GATA3, GATA binding protein 3, HDR, MGC2346, MGC5199, MGC5445, GATA-binding protein 3, enhancer-binding protein GATA3

Gene ID: 2625, 14462

NCBI Accession: NP_001002295, NP_002042

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation