SPRY1 antibody (C-Term)

Catalog No. ABIN570913

This Goat Polyclonal antibody specifically detects SPRY1 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for SPRY1 antibody (C-Term) (ABIN570913)

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SPRY1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SPRY1 Antibody

Binding Specificity: C-Term

Purpose: Sprouty

Sequence: CPSRGQGKPS

Specificity: NP_005832.1 and NP_955359.1 represent varients of the same protein.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Kidney lysates (calculated MW of 35.1 kDa according to NP_005832.1 ). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Rec

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SPRY1

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Alternative Name: SPRY1

Background: SPRY1, hSPRY1, sprouty homolog 1, antagonist of FGF signaling (Drosophila), sprouty (Drosophila) homolog 1 (antagonist of FGF signaling), sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)

Gene ID: 10252

NCBI Accession: NP_005832, NP_955359

Pathways: EGFR Signaling Pathway, EGFR Downregulation