WIZ antibody (Internal Region)

Catalog No. ABIN570947

The Goat Polyclonal anti-WIZ antibody is suitable to detect WIZ in samples from Human. It has been validated for WB, ELISA and FACS.

Quick Overview for WIZ antibody (Internal Region) (ABIN570947)

Target: WIZ (ZNF803) (Zinc Finger Protein 803 (ZNF803))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This WIZ antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-WIZ Antibody

Binding Specificity: Internal Region

Purpose: WIZ

Sequence: RSYIQGGRPF TKKFR

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-RSYIQGGRPFTKKFR

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 85.4 kDa according to NP_067064.2). An additional band of unknown identity was also consistently observed at 38 kDa. This band was successfully blocked by incuba

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for WIZ

Target: WIZ (ZNF803) (Zinc Finger Protein 803 (ZNF803))

Alternative Name: WIZ

Background: WIZ, widely interspaced zinc finger motifs, ZNF803, WIZ zinc finger, widely-interspaced zinc finger motifs

Gene ID: 58525, 22404, 314598

NCBI Accession: NP_067064, NP_001317324