SFRP4 antibody (Internal Region)

Catalog No. ABIN570948

This anti-SFRP4 antibody is a Goat Polyclonal antibody detecting SFRP4 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for SFRP4 antibody (Internal Region) (ABIN570948)

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SFRP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SFRP4 Antibody

Binding Specificity: Internal Region

Purpose: SFRP4

Sequence: TNSSCQCPHI LPHQD

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TNSSCQCPHILPHQD, from the internal region of the protein sequence according to NP_003005.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx 40 kDa band observed in Human Uterus lysates (calculated MW of 39.8 kDa according to NP_003005.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SFRP4

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Alternative Name: SFRP4

Background: SFRP4, secreted frizzled-related protein 4, FRP-4, FRPHE, MGC26498, secreted frizzled-related protein 4

Molecular Weight: 39.8kDa

Gene ID: 6424, 20379, 89803

NCBI Accession: NP_003005

Pathways: WNT Signaling