SLC47A1 antibody (Internal Region)

Catalog No. ABIN570957

This anti-SLC47A1 antibody is a Goat Polyclonal antibody detecting SLC47A1 in WB, ELISA and IF. Suitable for Human.

Quick Overview for SLC47A1 antibody (Internal Region) (ABIN570957)

Target: SLC47A1 (Solute Carrier Family 47, Member 1 (SLC47A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SLC47A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-SLC47A1 Antibody

Binding Specificity: Internal Region

Purpose: SLC47A1

Sequence: PEHPQDGAKL SRKQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PEHPQDGAKLSRKQ, from the internal region (near C Terminus) of the protein sequence according to NP_060712.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 60-65 kDa band observed in Human Liver lysates (calculated MW of 61.9 kDa according to NP_060712.2).This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-0.3 μg/mL. Primary incuba

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane of HepG2 cells. Recommened concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SLC47A1

Target: SLC47A1 (Solute Carrier Family 47, Member 1 (SLC47A1))

Alternative Name: SLC47A1

Background: SLC47A1, solute carrier family 47, member 1, FLJ10847, MATE1, MGC64822, multidrug and toxin extrusion 1

Gene ID: 55244

NCBI Accession: NP_060712