TSPO antibody (C-Term)

Catalog No. ABIN570960

This anti-TSPO antibody is a Goat Polyclonal antibody detecting TSPO in WB, ELISA, IHC and FACS. Suitable for Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for TSPO antibody (C-Term) (ABIN570960)

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TSPO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-TSPO Antibody

Binding Specificity: C-Term

Purpose: PBR (mouse)

Sequence: RDNSGRRGGS RLPE

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RDNSGRRGGSRLPE, from the C Terminus of the protein sequence according to NP_033905.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Mouse Kidney and Colon. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx.16-17 kDa band observed in Mouse Kidney lysates and in lysates of cell line NIH3T3 (calculated MW of 18.8 kDa according to NP_033905.3). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-TSPO antibody (ABIN570960)

Morgan, Cheepala, Wang, Neale, Adachi, Nachagari, Leggas, Zhao, Boyd, Venkataramanan, Schuetz: "Deregulated hepatic metabolism exacerbates impaired testosterone production in Mrp4-deficient mice." in: The Journal of biological chemistry, Vol. 287, Issue 18, pp. 14456-66, (2012) (PubMed).

Target Details for TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Alternative Name: Tspo

Background: PBR, Tspo, translocator protein, Bzrp, IBP, benzodiazepine receptor, peripheral, isoquinoline-binding protein, peripheral benzodiazepine receptor

Gene ID: 12257, 24230

NCBI Accession: NP_033905

Pathways: Cellular Response to Molecule of Bacterial Origin