SH2D3A antibody (Internal Region)

Catalog No. ABIN570964

This Goat Polyclonal antibody specifically detects SH2D3A in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for SH2D3A antibody (Internal Region) (ABIN570964)

Target: SH2D3A (SH2 Domain Containing 3A (SH2D3A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SH2D3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SH2D3A Antibody

Binding Specificity: Internal Region

Purpose: SH2D3A / NSP1

Sequence: APRAERFEKF QR

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-APRAERFEKFQR, from the internal region (near C terminus) of the protein sequence according to NP_005481.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 5 μg/mL.

Western Blot: Approx. 60 kDa band observed in Human Lung and Tonsil lysates (calculated MW of 63.1 kDa according to NP_005481.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SH2D3A

Target: SH2D3A (SH2 Domain Containing 3A (SH2D3A))

Alternative Name: SH2D3A

Background: SH2D3A, NSP1, SH2 domain containing 3A, SH2 domain-containing 3A, novel SH2-containing protein 1

Gene ID: 10045

NCBI Accession: NP_005481