Unc5c antibody (Internal Region)

Catalog No. ABIN570965

The Goat Polyclonal anti-Unc5c antibody has been validated for ELISA, IHC and IF. It is suitable to detect Unc5c in samples from Human and Mouse.

Quick Overview for Unc5c antibody (Internal Region) (ABIN570965)

Target: Unc5c (Unc-5 Homolog C (C. Elegans) (Unc5c))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Unc5c antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Unc5c Antibody

Binding Specificity: Internal Region

Purpose: UNC5C

Sequence: NCTVSEEPTG ID

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence NCTVSEEPTGID, from the internal region of the protein sequence according to NP_003719.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of some neuronal bodies plus intense axonal staining. Recommended concentration: 3-5 μg/mL.

Western Blot: Preliminary experiments in Human and Mouse Brain lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL).

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Staining of paraffin embedded endocrine islets (red) in adult Mouse Pancreas. Nuclear staining in blue and ductal cell staining in green. Data obtained from a customer.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Unc5c

Target: Unc5c (Unc-5 Homolog C (C. Elegans) (Unc5c))

Alternative Name: UNC5C

Background: UNC5C, unc-5 homolog C (C. elegans), UNC5H3, homolog of C. elegans transmembrane receptor Unc5, unc5 (C.elegans homolog) c, unc5C

Gene ID: 8633, 22253

NCBI Accession: NP_003719