IGFBP7 antibody (AA 145-159)

Catalog No. ABIN570977

The Goat Polyclonal anti-IGFBP7 antibody has been validated for WB and ELISA. It is suitable to detect IGFBP7 in samples from Mouse.

Quick Overview for IGFBP7 antibody (AA 145-159) (ABIN570977)

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IGFBP7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IGFBP7 Antibody

Binding Specificity: AA 145-159

Purpose: IGFBP7 (aa145-159)

Sequence: EKAITQVSKG TCEQG

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence EKAITQVSKGTCEQG, from the internal region of the protein sequence according to NP_001544.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Mouse Kidney lysates (calculated MW of 29.1 kDa according to Human NP_001544.1 and to Mouse NP_032074.3). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IGFBP7

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Alternative Name: IGFBP7

Background: IGFBP7, insulin-like growth factor binding protein 7, FSTL2, IGFBP-7, IGFBP-7v, IGFBPRP1, MAC25, PSF

Gene ID: 3490

NCBI Accession: NP_001544

Pathways: Growth Factor Binding