MEST antibody (Internal Region)

Catalog No. ABIN570981

This anti-MEST antibody is a Goat Polyclonal antibody detecting MEST in ELISA and WB. Suitable for Mouse and Rat.

Quick Overview for MEST antibody (Internal Region) (ABIN570981)

Target: MEST (Mesoderm Specific Transcript (MEST))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This MEST antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-MEST Antibody

Binding Specificity: Internal Region

Purpose: MEST

Sequence: QELLYRYKQN RSGR

Specificity: This antibody is expected to recognize both reported isoforms (NP_002393.2, NP_803490.1). Reported variants NP_803490.1 and NP_803491.1 represent identical protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QELLYRYKQNRSGR, from the internal region of the protein sequence according to NP_002393.2, NP_803490.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in Mouse and Rat Testis lysates (calculated MW of 37.6 kDa according to Human NP_803490.1 and 38.8 kDa according to Rat NP_001009617.1). Recommended concentration: 0.2-0.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MEST

Target: MEST (Mesoderm Specific Transcript (MEST))

Alternative Name: MEST

Background: MEST, mesoderm specific transcript homolog (mouse), DKFZp686L18234, MGC111102, MGC8703, PEG1, mesoderm specific transcript, paternally expressed gene 1

Gene ID: 4232, 17294, 58827

NCBI Accession: NP_002393, NP_803490