CaMKII alpha/beta (Internal Region) antibody
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- Target
- CaMKII alpha/beta
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Binding Specificity
- Internal Region
- Reactivity
- Rat, Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- CAMK2
- Sequence
- PRTAQSEETR VWHR
- Specificity
- This antibody is expected to recognize both reported isoforms (NP_057065.2 (CAMK2A) and NP_001280099.1 (CAMK2B).
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2, NP_741960.1.
- Isotype
- IgG
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anti-CaMKII alpha/beta (pThr286) antibody
Reactivity: Rat WB Host: Rabbit Polyclonal unconjugated
anti-CaMKII alpha/beta (pThr306) antibodyReactivity: Rat WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western Blot: Approx 48-50+60 kDa bands observed in Human Cerebral Cortex and Mouse and Rat Brain lysates, corresponding to CAMK2alpha and CAMK2beta respectively and approx. 60 kDa in Human Cerebellum lysates (calculated MW of 55.3 kDa according to Human NP
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of Neuro2a cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Neuro2a cells. Recommended concentration: 10u
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- CaMKII alpha/beta
- Alternative Name
- CAMK2A/B
- Background
- CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase
- Gene ID
- 815, 816, 12322
- NCBI Accession
- NP_057065, NP_741960
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