ACPP antibody (Internal Region)

Catalog No. ABIN570986

The Goat Polyclonal anti-ACPP antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect ACPP in samples from Human and Mouse. There is 1 publication available.

Quick Overview for ACPP antibody (Internal Region) (ABIN570986)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ACPP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-ACPP Antibody

Binding Specificity: Internal Region

Purpose: ACPP / PAP

Sequence: YGIHKQKEKS RLQ

Specificity: This antibody is expected to recognize both isoforms (NP_001090.2, NP_001127666.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-YGIHKQKEKSRLQ

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5 μg/mL. This antibody has been successfully used in IHC on Mouse: Nousiainen HO et al. (2014) PMID: 2484

Western Blot: Approx 50 kDa band observed in Human Prostate lysates (calculated MW of 44.6 kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, ,Clin Chem. 1986 O

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence:: This antibody has been successfully used in IF on Mouse: Nousiainen HO et al. (2014) PMID: 24846136.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ACPP antibody (ABIN570986)

Nousiainen, Quintero, Myöhänen, Voikar, Mijatovic, Segerstråle, Herrala, Kulesskaya, Pulkka, Kivinummi, Abo-Ramadan, Taira, Piepponen, Rauvala, Vihko: "Mice deficient in transmembrane prostatic acid phosphatase display increased GABAergic transmission and neurological alterations." in: PLoS ONE, Vol. 9, Issue 5, pp. e97851, (2014) (PubMed).

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACP3

Background: ACPP, acid phosphatase, prostate, ACP-3, ACP3, PAP, prostatic acid phosphotase

Gene ID: 55, 56318, 56780

NCBI Accession: NP_001090

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process