PPP4C antibody (C-Term)

Catalog No. ABIN570988

This Goat Polyclonal antibody specifically detects PPP4C in WB, IHC and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for PPP4C antibody (C-Term) (ABIN570988)

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP4C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PPP4C Antibody

Binding Specificity: C-Term

Purpose: PPP4C

Sequence: PQETRGIPSK KP

Cross-Reactivity: Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PQETRGIPSKKP, from the C Terminus of the protein sequence according to NP_002711.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx 35 kDa band observed in HEK293, A431 and HeLa lysates and in Rat Spleen and Lung lysates (calculated MW of 35.1 kDa according to NP_002711.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP4C

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Alternative Name: PPP4C

Background: PPP4C, protein phosphatase 4 (formerly X), catalytic subunit, PP4, PPH3, PPX, Protein phosphatase 4, catalytic subunit

Gene ID: 5531, 171366

NCBI Accession: NP_002711