PLA2R1 antibody (Internal Region)

Catalog No. ABIN570997

This Goat Polyclonal antibody specifically detects PLA2R1 in ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for PLA2R1 antibody (Internal Region) (ABIN570997)

Target: PLA2R1 (phospholipase A2 Receptor 1, 180kDa (PLA2R1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PLA2R1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PLA2R1 Antibody

Binding Specificity: Internal Region

Purpose: PLA2R1

Sequence: EKKKDTPKQH GT

Specificity: This antibody is expected to recognize all reported isoforms (NP_031392.3, NP_001007268.1, NP_001182570.1).

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKKKDTPKQHGT, from the Internal region of the protein sequence according to NP_031392.3, NP_001007268.1, NP_001182570.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 150 kDa in HeLa cell lysate after 2 μg/mL antibody staining (calculated MW of 153 kDa according to NP_001007268.1 Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PLA2R1

Target: PLA2R1 (phospholipase A2 Receptor 1, 180kDa (PLA2R1))

Alternative Name: PLA2R1

Background: PLA2R1, phospholipase A2 receptor 1, 180 kDa, CLEC13C, PLA2-R, PLA2G1R, PLA2IR, PLA2R, phospholipase A2 receptor 1

Gene ID: 22925

NCBI Accession: NP_031392, NP_001007268, NP_001182570

Pathways: Positive Regulation of Response to DNA Damage Stimulus