Decorin antibody (Internal Region)

Catalog No. ABIN571007

The Goat Polyclonal anti-Decorin antibody has been validated for WB, ELISA and IHC. It is suitable to detect Decorin in samples from Human.

Quick Overview for Decorin antibody (Internal Region) (ABIN571007)

Target: Decorin (DCN)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Decorin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Decorin Antibody

Binding Specificity: Internal Region

Purpose: Decorin

Sequence: KISRVDAASL KGLNN

Specificity: This antibody is expected to recognize the three reported isoforms a, b and c (NP_001911.1, NP_598011.1, NP_598012.1 resp.). Reported variants NP_598010.1 and NP_001911.1 represent identical protein.

Cross-Reactivity: Cow, Guinea Pig, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KISRVDAASLKGLNN, from the internal region of the protein sequence according to NP_001911.1, NP_598011.1, NP_598012.1,.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 38 kDa band observed in Human Kidney lysates (calculated MW of 39.7 kDa according to NP_001911.1). Recommended concentration: 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: DCN

Background: DCN, decorin, CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B, bone proteoglycan II, decorin proteoglycan, dermatan sulphate proteoglycans II, proteoglycan core protein, small leucine-rich protein 1B

Gene ID: 1634

NCBI Accession: NP_001911, NP_598011, NP_598012

Pathways: Glycosaminoglycan Metabolic Process