ITGAL antibody (Internal Region)

Catalog No. ABIN571017

The Goat Polyclonal anti-ITGAL antibody (ABIN571017) specifically detects ITGAL in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for ITGAL antibody (Internal Region) (ABIN571017)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ITGAL Antibody

Binding Specificity: Internal Region

Purpose: CD11A / integrin alpha L

Sequence: YQVRIQPSIH DHN

Specificity: This antibody is expected to recognize both reported isoforms (NP_002200.2, NP_001107852.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YQVRIQPSIHDHN, from the internal region of the protein sequence according to NP_002200.2, NP_001107852.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 200 kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129 kDa according to NP_002200.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: ITGAL

Background: ITGAL, integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide), CD11A, LFA-1, LFA1A, LFA-1 alpha, antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide, integrin alpha L, integrin g

Molecular Weight: 129kDa

Gene ID: 3683

NCBI Accession: NP_002200, NP_001107852

Pathways: Activated T Cell Proliferation, Integrin Complex