NFAT1 antibody (Internal Region)

Catalog No. ABIN571020

This anti-NFAT1 antibody is a Goat Polyclonal antibody detecting NFAT1 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for NFAT1 antibody (Internal Region) (ABIN571020)

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NFAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-NFAT1 Antibody

Binding Specificity: Internal Region

Purpose: NFATC2 / NFAT1

Sequence: QQFRTGLSSP DARYQ

Specificity: This antibody is expected to recognize all three reported isoforms (NP_036472.2, NP_775114.1, NP_001129493.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QQFRTGLSSPDARYQ, from the internal region of the protein sequence according to NP_036472.2, NP_775114.1, NP_001129493.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear membrane and weaker cytoplasm staining in select splenocytes. Recommended concentration, 3-6 μg/mL.

Western Blot: Approx 110 kDa band observed in Human lysates of cell line MOLT4 (calculated MW of 100 kDa according to NP_775114.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NFAT1

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Alternative Name: NFATC2

Background: NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, KIAA0611, NFAT1, NFATP, NFAT pre-existing subunit, NFAT transcription complex, preexisting component, OTTHUMP00000031291, T cell transcription factor NFAT1, nuclear factor

Gene ID: 4773, 140488, 312320

NCBI Accession: NP_036472, NP_775114, NP_001129493

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling, BCR Signaling