CD158a/g/h antibody (PE)

Catalog No. ABIN5710220

The Mouse Monoclonal anti- antibody has been validated for FACS. It is suitable to detect in samples from Human.

Quick Overview for CD158a/g/h antibody (PE) (ABIN5710220)

Target: CD158a/g/h

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS)

Clone: HP-MA4

Product Details

Purpose: Anti-Hu CD158agh PE

Specificity: The mouse monoclonal antibody HP-MA4 recognizes an extracellular epitope of CD158 isoforms KIR2DL1 (CD158a), KIR2DS5 (CD158g), KIR2DS1 (CD158h), and KIR2DS3. It does not recognize the isoforms CD158b1,d,f,i,j.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human NK cell line LB2

Isotype: IgG2b kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details

Target: CD158a/g/h

Alternative Name: CD158agh

Background: Killer cell immunoglobulin like receptor, two Ig d,Killer cell immunoglobulin-like receptors (KIRs) are polymorphic transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. They are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain (such as CD158a / KIR2DL1) transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain (such as CD158g / KIR2DS5, CD158h / KIR2DS1, or KIR2DS3) lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for CD158 isoforms are subsets of MHC class I molecules.,KIR2DL1, NKAT, KIR2DS1, KIR2DS3, NKAT7, KIR2DS5, NKAT9, CD158g, CD158h, CD158a

Gene ID: 3805

UniProt: Q99706