SRF antibody (Internal Region)

Catalog No. ABIN571032

The Goat Polyclonal anti-SRF antibody (ABIN571032) specifically detects SRF in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for SRF antibody (Internal Region) (ABIN571032)

Target: SRF (Serum Response Factor (SRF))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SRF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SRF Antibody

Binding Specificity: Internal Region

Purpose: SRF

Sequence: QASPSRDSST DLTQ

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QASPSRDSSTDLTQ, from the internal region of the protein sequence according to NP_003122.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 67 kDa band observed in primary cultured Human Lung fibroblasts lysates after 24 h treatment with 1 ng/mL TGF-bl (calculated MW of 51.6 kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in lit

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SRF

Target: SRF (Serum Response Factor (SRF))

Alternative Name: SRF

Background: SRF, serum response factor (c-fos serum response element-binding transcription factor), MCM1, OTTHUMP00000039820

Gene ID: 6722, 20807, 501099

NCBI Accession: NP_003122