CA1 antibody (Internal Region)

Catalog No. ABIN571033

This Goat Polyclonal antibody specifically detects CA1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CA1 antibody (Internal Region) (ABIN571033)

Target: CA1 (Carbonic Anhydrase I (CA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CA1 Antibody

Binding Specificity: Internal Region

Purpose: CA1

Sequence: QAIKTKGKRA P

Specificity: Reported variants represent identical protein: NP_001729.1, NP_001122303.1, NP_001122302.1, NP_001122301.1.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QAIKTKGKRAP

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 29 kDa band observed in Human Liver lysates (calculated MW of 28.9 kDa according to NP_001729.1. Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CA1

Target: CA1 (Carbonic Anhydrase I (CA1))

Alternative Name: CA1

Background: CA1, carbonic anhydrase I, Car1, carbonic dehydratase

Gene ID: 759, 12346, 310218

NCBI Accession: NP_001729