CNN2 antibody (Internal Region)

Catalog No. ABIN571041

This anti-CNN2 antibody is a Goat Polyclonal antibody detecting CNN2 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for CNN2 antibody (Internal Region) (ABIN571041)

Target: CNN2 (Calponin 2 (CNN2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CNN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CNN2 Antibody

Binding Specificity: Internal Region

Purpose: Calponin 2 / CNN2

Sequence: DPGEVPEYPP YYQEE

Specificity: This antibody is expected to recognize both reported isoforms (NP_004359.1, NP_958434.1), but it is not expected to cross-react with CCN3.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DPGEVPEYPPYYQEE, from the internal region (near C Terminus) of the protein sequence according to NP_004359.1, NP_958434.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 35 kDa band observed in lysates of cell line HepG2 (calculated MW of 33.7 kDa according to NP_004359.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CNN2

Target: CNN2 (Calponin 2 (CNN2))

Alternative Name: CNN2

Background: CNN2, calponin 2, calponin H2, smooth muscle, neutral calponin

Gene ID: 1265

NCBI Accession: NP_004359, NP_958434