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CD3 antibody

Name: CD3 antibody
Brand: antibodies-online
SKU: ABIN5710579
Price: 387.09 USD
Availability: InStock

CD3 Reactivity: Human FACS, ICC Host: Mouse Monoclonal TB3 unconjugated

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (TB3) purified antibody (concentration in sample 0,3 μg/mL) GAM APC. (CD3 antibody)

FACS

Surface staining of human buffy coat cells with anti-CD3 (TB3) purified / GAM-APC. (CD3 antibody)

FACS

Immunocytochemistry staining of human CD3 epsilon and CD3 gamma transfected COS cells using mouse monoclonal antibodies anti-CD3, clones UCHT1 (left) and TB3 (right) indicated by Alexa Fluor 555 signal (red), DNA stained by DAPI (blue). (CD3 antibody)

ICC

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (TB3) purified antibody (concentration in sample 0,3 μg/mL) GAM APC. (CD3 antibody)

FACS

4 images

Catalog No. ABIN5710579

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
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102

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20

17

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8

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7

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3

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1
Human
Host
212

53

20

13

9

2
Mouse
Compatible Secondaries
Clonality
297

6

1
Monoclonal
Conjugate
94

38

30

23

23

11

9

8

6

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5

5

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4

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1

1

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This CD3 antibody is un-conjugated
Application
282

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46

35

33

22

9

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Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity

The mouse monoclonal antibody TB3 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody has superior binding than the clone TB2.

Cross-Reactivity (Details)

Human

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Clone

TB3

Isotype

IgG2b

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anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human FACS Host: Mouse Monoclonal MEM-57 PerCP

anti-CD3 (CD3) antibody (APC)
CD3 Reactivity: Human FACS Host: Mouse Monoclonal MEM-57 APC

Application Notes

Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Target

CD3

Alternative Name

CD3 (CD3 Products)

Background

CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

Gene ID

916

UniProt

P07766

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway