Neurogranin antibody (Internal Region)

Catalog No. ABIN571065

This anti-Neurogranin antibody is a Goat Polyclonal antibody detecting Neurogranin in WB, IHC and ELISA. Suitable for Human.

Quick Overview for Neurogranin antibody (Internal Region) (ABIN571065)

Target: Neurogranin (NRGN)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Neurogranin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Neurogranin Antibody

Binding Specificity: Internal Region

Purpose: Neurogranin precursor

Sequence: KIKSGERGRK G

Specificity: Reported variants represent identical protein: NP_006167.1, NP_001119653.1

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KIKSGERGRKG, from the internal region of the protein sequence according to NP_006167.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 16 kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 7.6 kDa according to NP_006167.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Ch

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Neurogranin

Target: Neurogranin (NRGN)

Alternative Name: NRGN

Background: NRGN, neurogranin (protein kinase C substrate, RC3), RC3, hng, calmodulin-binding protein, neurogranin, protein kinase C substrate

Molecular Weight: 7.6kDa

Gene ID: 4900, 647011

NCBI Accession: NP_006167