PRDM1 antibody (Internal Region)

Catalog No. ABIN571072

This Goat Polyclonal antibody specifically detects PRDM1 in WB, ELISA, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for PRDM1 antibody (Internal Region) (ABIN571072)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRDM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-PRDM1 Antibody

Binding Specificity: Internal Region

Purpose: PRDM1 / MEL1

Sequence: DISDNADRLE DVED

Specificity: This antibody is expected to recognize both reported isoforms (NP_001189.2, NP_878911.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DISDNADRLEDVED, from the internal region of the protein sequence according to NP_001189.2, NP_878911.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 6 μg/mL.

Western Blot: Approx 90 kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100 kDa in lysates of cell line K562. An additional faint band of approx 70 kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8 kDa accor

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: PRDM1

Background: PRDM1, PR domain containing 1, with ZNF domain, BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1, B-lymphocyte-induced maturation protein 1, OTTHUMP00000016918, PR-domain zinc finger protein 1, PRDI-binding factor-1, beta-interferon gene posit

Gene ID: 639, 12142, 309871

NCBI Accession: NP_001189, NP_878911

Pathways: Regulation of Muscle Cell Differentiation