STAG2 antibody (Internal Region)

Catalog No. ABIN571073

The Goat Polyclonal anti-STAG2 antibody has been validated for WB and ELISA. It is suitable to detect STAG2 in samples from Human, Mouse and Rat.

Quick Overview for STAG2 antibody (Internal Region) (ABIN571073)

Target: STAG2 (Stromal Antigen 2 (STAG2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This STAG2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-STAG2 Antibody

Binding Specificity: Internal Region

Purpose: Stromal antigen 2 / STAG2

Sequence: SRGSTVRSKK SKPST

Specificity: This antibody is expected to recognize both reported isoforms (NP_001036214.1, NP_006594.3). Reported variants NP_001036215.1 and NP_001036214.1 represent identical protein: Reported variants NP_001036216.1 and NP_006594.3 represent identical protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SRGSTVRSKKSKPST, from the internal region of the protein sequence according to NP_001036214.1, NP_006594.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 160 kDa band observed in lysates of cell line K562 and ~140 kDa in rodent Spleen while ~150 kDa in Mouse Thymus lysates (calculated MW of 146 kDa according to Human NP_001036214.1 and 140 kDa according to Mouse NP_001071180.1). Recommen

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for STAG2

Target: STAG2 (Stromal Antigen 2 (STAG2))

Alternative Name: STAG2

Background: STAG2, stromal antigen 2, DKFZp686P168, DKFZp781H1753, FLJ25871, SA-2, SA2, bA517O1.1, OTTHUMP00000024338, OTTHUMP00000043514, OTTHUMP00000043515, OTTHUMP00000081754, OTTHUMP00000081755, SCC3 homolog 2

Gene ID: 10735, 20843, 313304

NCBI Accession: NP_001036214, NP_006594

Pathways: Stem Cell Maintenance