ACAT1 antibody (AA 257-269)

Catalog No. ABIN571082

The Goat Polyclonal anti-ACAT1 antibody has been validated for WB, ELISA and IF. It is suitable to detect ACAT1 in samples from Human, Mouse and Rat.

Quick Overview for ACAT1 antibody (AA 257-269) (ABIN571082)

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ACAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-ACAT1 Antibody

Binding Specificity: AA 257-269

Purpose: ACAT1 (aa257-269)

Sequence: KRVDFSKVPK LKT

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KRVDFSKVPKLKT, from the internal region of the protein sequence according to NP_000010.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in Human Liver lysates and in rodent Kidney and Liver lysates (calculated MW of 45.2 kDa according to NP_000010.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Customer finds particulate cytoplasm staining in HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: ACAT1

Background: ACAT1, acetyl-Coenzyme A acetyltransferase 1, ACAT, MAT, T2, THIL, acetoacetyl Coenzyme A thiolase

Gene ID: 38, 110446, 25014

NCBI Accession: NP_000010