ACAT1 antibody (AA 253-266)

Catalog No. ABIN571083

This anti-ACAT1 antibody is a Goat Polyclonal antibody detecting ACAT1 in WB, ELISA, IF and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for ACAT1 antibody (AA 253-266) (ABIN571083)

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ACAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ACAT1 Antibody

Binding Specificity: AA 253-266

Purpose: ACAT1 (aa253-266)

Sequence: DEEYKRVDFS KVPK

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DEEYKRVDFSKVPK, from the internal region of the protein sequence according to NP_000010.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 40 kDa band observed in lysates of cell lines CAC02, HEK293, HepG2, MCF7, NIH3T3 and KNRK and approx. 38-40 kDa in Human, Mouse and Rat Liver lysates (calculated MW of 45.2 kDa according to Human NP_000010.1, 44.81 kDa according to Mous

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: ACAT1

Background: ACAT1, acetyl-Coenzyme A acetyltransferase 1, ACAT, MAT, T2, THIL, acetoacetyl Coenzyme A thiolase

Gene ID: 38, 110446, 25014

NCBI Accession: NP_000010