alpha Defensin 1 antibody (Internal Region)

Catalog No. ABIN571084

This Goat Polyclonal antibody specifically detects alpha Defensin 1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for alpha Defensin 1 antibody (Internal Region) (ABIN571084)

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha Defensin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-alpha Defensin 1 Antibody

Binding Specificity: Internal Region

Purpose: DEFA1/3 precursor

Sequence: DESLAPKHPG SRKN

Specificity: This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DESLAPKHPGSRKN, from the internal region of the protein sequence according to NP_004075.1, NP_005208.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thymus. Recommended concentration: 3-6 μg/mL.

Western Blot: Approx 10 kDa band observed in Human Spleen lysates (calculated MW of 10.2 kDa according to NP_004075.1). An additional band of unknown identity was also consistently observed at 20 kDa, and was successfully blocked by incubation with the imm

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for alpha Defensin 1

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Alternative Name: DEFA1

Background: DEFA1, defensin, alpha 1, myeloid-related sequence , HGNC:2761, DEF1, DEFA2, HNP-1, HP-1, MRS , defensin, alpha 1, defensin, alpha 2, myeloid-related sequence, DEFA3, defensin, alpha 3, neutrophil-specific, DEF3, HNP-3, HNP3, HP-3, defensin 3, neutrophil-

Molecular Weight: 10.2kDa

Gene ID: 1667, 1668

NCBI Accession: NP_004075, NP_005208

Pathways: Cellular Response to Molecule of Bacterial Origin