Sorting Nexin 1 antibody (Internal Region)

Catalog No. ABIN571095

This anti-Sorting Nexin 1 antibody is a Goat Polyclonal antibody detecting Sorting Nexin 1 in WB, ELISA, IHC and ICC. Suitable for Human.

Quick Overview for Sorting Nexin 1 antibody (Internal Region) (ABIN571095)

Target: Sorting Nexin 1 (SNX1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Sorting Nexin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-Sorting Nexin 1 Antibody

Binding Specificity: Internal Region

Purpose: SNX1

Sequence: TNSSKPQPTY EELE

Specificity: This antibody is expected to recognize isoform a and c (NP_003090.2, NP_690039.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TNSSKPQPTYEELE, from the internal region of the protein sequence according to NP_003090.2, NP_690039.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Uterus shows vesicular staining in the cytoplasm of endometrial epithelium. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell lines HeLa and U2OS (calculated MW of 59.1 kDa according to NP_003090.2). This molecular weight is routinely observed by other sources . Recommended concentration: 0.1-1 μg/mL. Primary incubation

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunocytochemistry: Vesicular staining of SNX1 (green) in formaldehyde-fixed SH5Y5Y. Recommended concentration, 3-5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Sorting Nexin 1

Target: Sorting Nexin 1 (SNX1)

Alternative Name: SNX1

Background: SNX1, sorting nexin 1, HsT17379, MGC8664, SNX1A, Vps5, sorting nexin 1A

Gene ID: 6642, 56440, 84471

NCBI Accession: NP_003090, NP_690039