TGFBI antibody (Internal Region)

Catalog No. ABIN571098

This anti-TGFBI antibody is a Goat Polyclonal antibody detecting TGFBI in WB, IHC, ELISA and IF. Suitable for Human and Rat.

Quick Overview for TGFBI antibody (Internal Region) (ABIN571098)

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This TGFBI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-TGFBI Antibody

Binding Specificity: Internal Region

Purpose: TGFBI

Sequence: QLYTDRTEKL RPE

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QLYTDRTEKLRPE, from the internal region of the protein sequence according to NP_000349.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration: 4-6 μg/mL. This antibody has been successfully used in IHC on Rat, PMID: 26407577.

Western Blot: Approx 70 kDa band observed in Human and Rat Kidney lysates, and approx 75 kDa in Human Prostate and Human Colorectal cancer lysates (calculated MW of 74.7 kDa according to Human NP_000349.1 and 74.8 kDa according to Rat NP_446254.1). Recommend

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the golgi and plasma membrane of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TGFBI

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Alternative Name: TGFBI

Background: TGFBI, transforming growth factor, beta-induced, 68 kDa, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1, RGD-containing collagen-associated protein, kerato-epithelin

Molecular Weight: 74.7kDa

Gene ID: 7045, 21810, 24511

NCBI Accession: NP_000349