PON2 antibody (Internal Region)

Catalog No. ABIN571113

This anti-PON2 antibody is a Goat Polyclonal antibody detecting PON2 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for PON2 antibody (Internal Region) (ABIN571113)

Target: PON2 (Paraoxonase 2 (PON2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PON2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PON2 Antibody

Binding Specificity: Internal Region

Purpose: PON2

Sequence: EKPRARELRI S

Specificity: This antibody is expected to recognize isoforms 1 and 2 (NP_000296.2, NP_001018171.1).

Cross-Reactivity: Dog, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKPRARELRIS, from the internal region of the protein sequence according to NP_000296.2, NP_001018171.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa double band observed in lysates of HepG2 (calculated MW of 39.4 kDa according to NP_000296.2 and of 38.0 kDa according to NP_001018171.1). Recommended concentration: 0.2-0.6 μg/mL. Primary incubation was 1 hour. An additional band

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PON2

Target: PON2 (Paraoxonase 2 (PON2))

Alternative Name: PON2

Background: PON2, paraoxonase 2, A-esterase 2, aromatic esterase 2, paraoxonase nirs, serum aryldialkylphosphatase 2, serum paraoxonase/arylesterase 2

Gene ID: 5445, 296851

NCBI Accession: NP_000296, NP_001018171