CHCHD3 antibody (AA 151-164)

Catalog No. ABIN571117

This Goat Polyclonal antibody specifically detects CHCHD3 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for CHCHD3 antibody (AA 151-164) (ABIN571117)

Target: CHCHD3 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3 (CHCHD3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CHCHD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CHCHD3 Antibody

Binding Specificity: AA 151-164

Purpose: CHCHD3 (aa151-164)

Sequence: RSSEFYRVTT EQYQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Sheep

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RSSEFYRVTTEQYQ, from the internal region of the protein sequence according to NP_060282.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 26.2 kDa band observed in Human Placenta lysates (calculated MW of 26 kDa according to NP_060282.1). Recommended concentration: 0.3-1 μg/mL. A customer reported ~30 kDa in THP1 and in human primary monocyte derived dendritic cells.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHCHD3

Target: CHCHD3 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3 (CHCHD3))

Alternative Name: CHCHD3

Background: CHCHD3, coiled-coil-helix-coiled-coil-helix domain containing 3, FLJ20420

Gene ID: 54927, 66075, 296966

NCBI Accession: NP_060282