CRISP2 antibody (Internal Region)

Catalog No. ABIN571121

The Goat Polyclonal anti-CRISP2 antibody has been validated for WB, IHC and ELISA. It is suitable to detect CRISP2 in samples from Human.

Quick Overview for CRISP2 antibody (Internal Region) (ABIN571121)

Target: CRISP2 (Cysteine-Rich Secretory Protein 2 (CRISP2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CRISP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CRISP2 Antibody

Binding Specificity: Internal Region

Purpose: CRISP2

Sequence: QHSDPEDRKT STR

Specificity: Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QHSDPEDRKTSTR

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 3.75 μg/mL.

Western Blot: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recomm

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CRISP2

Target: CRISP2 (Cysteine-Rich Secretory Protein 2 (CRISP2))

Alternative Name: CRISP2

Background: CRISP2, cysteine-rich secretory protein 2, CRISP-2, GAPDL5, MGC111136, TPX1, TSP1, OTTHUMP00000016589, glyceraldehyde-3-phosphate dehydrogenase-like 5, testis specific protein 1 (probe H4-1 p3-1)

Gene ID: 7180

NCBI Accession: NP_003287