LSP1 antibody (C-Term)

Catalog No. ABIN571130

The Goat Polyclonal anti-LSP1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect LSP1 in samples from Human and Mouse.

Quick Overview for LSP1 antibody (C-Term) (ABIN571130)

Target: LSP1 (Lymphocyte-Specific Protein 1 (LSP1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This LSP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-LSP1 Antibody

Binding Specificity: C-Term

Purpose: LSP1

Sequence: RYKFVATGHG KYEKV

Specificity: This antibody is expected to recognize both reported isoforms (NP_002330.1, NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RYKFVATGHGKYEKV, from the C Terminus of the protein sequence according to NP_002330.1, NP_001013271.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Colon, MALT. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Peripheral Blood Mononucleocytes, (PBMs) and Tonsil lysates and in lysates of cell line Daudi, and approx. 50-55 kDa in Human Tonsil and Mouse Spleen lysates (calculated MW of 37.2 kDa according to Human NP

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LSP1

Target: LSP1 (Lymphocyte-Specific Protein 1 (LSP1))

Alternative Name: LSP1

Background: LSP1, lymphocyte-specific protein 1, WP34, pp52, 47 kDa actin binding protein, 52 kDa phosphoprotein, F-actin binding and cytoskeleton associated protein, leufactin (leukocyte F-actin binding protein), leukocyte-specific protein 1, lymphocyte-specific ant

Gene ID: 4046, 16331, 54259

NCBI Accession: NP_002330, NP_001013271