POU3F2 antibody (Internal Region)

Catalog No. ABIN571160

The Goat Polyclonal anti-POU3F2 antibody has been validated for WB and ELISA. It is suitable to detect POU3F2 in samples from Human, Rat and Mouse.

Quick Overview for POU3F2 antibody (Internal Region) (ABIN571160)

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This POU3F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-POU3F2 Antibody

Binding Specificity: Internal Region

Purpose: POU3F2 / BRN2 / OCT7

Sequence: AQSLVQGDYG ALQSN

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-AQSLVQGDYGALQSN, from the internal region (near N Terminus) of the protein sequence according to NP_005595.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9 kDa according to Human NP_005595.2 and 47.1 kDa according to Mouse NP_032925.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for POU3F2

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Alternative Name: POU3F2

Background: POU3F2, POU class 3 homeobox 2, BRN2, OCT7, OTF7, POUF3, POU domain, class 3, transcription factor 2

Gene ID: 5454, 18992, 29588

NCBI Accession: NP_005595