POU3F3 antibody (Internal Region)

Catalog No. ABIN571161

This anti-POU3F3 antibody is a Goat Polyclonal antibody detecting POU3F3 in WB, ELISA, FACS and IF. Suitable for Mouse.

Quick Overview for POU3F3 antibody (Internal Region) (ABIN571161)

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This POU3F3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-POU3F3 Antibody

Binding Specificity: Internal Region

Purpose: POU3F3 / BRN1 / OCT8

Sequence: HMLSHAHQWV TAL

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Mouse Spinal Cord lysates and approx. 50 kDa in Mouse Brain lysates (calculated MW of 50.3 kDa according to Human NP_006227.1 and 50.2 kDa according to Mouse NP_032926.2). Recommended concentration: 1-3 μg/mL. Pri

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for POU3F3

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Alternative Name: POU3F3

Background: POU3F3, POU class 3 homeobox 3, BRN1, OTF8, OTF-8, POU domain, class 3, transcription factor 3, brain-1, brain-specific homeobox/POU domain protein 1, brn-1, oct-8, octamer-binding protein 8, octamer-binding transcription factor 8

Gene ID: 5455, 18993, 192109

NCBI Accession: NP_006227