POU3F3 antibody (AA 351-450)

Catalog No. ABIN1386623

The Rabbit Polyclonal anti-POU3F3 antibody is suitable to detect POU3F3 in samples from Human. It has been validated for WB, IF (cc), IF (p), IHC (fro) and IHC (p).

Quick Overview for POU3F3 antibody (AA 351-450) (ABIN1386623)

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU3F3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POU3F3 Antibody

Binding Specificity: AA 351-450

Purpose: POU3F3 Polyclonal Antibody

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Brain1/POU3F3

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000),

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for POU3F3

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Alternative Name: Brain1/POU3F3

Background:

Synonyms: Brain 1, Brain specic homeobox/POU domain protein 1, Brain-1, Brain-specic homeobox/POU domain protein 1, Brain1, BRN 1, Brn-1, BRN1, class 3, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, OTF 8, OTF-8, OTF8, PO3 F3, PO3F 3, PO3F3, PO3F3_HUMAN, POU class 3 homeobox 3, POU domain, POU domain, class 3, transcription factor 3, POU3 F3, POU3F 3, Pou3f3, RHS 1, Rhs 2, RHS1, Rhs2, Skin 1, Skin1, transcription factor 3.

Background: The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU domain proteins. Expressed during the development of the forebrain and coexpressed in most layer II-V cortical neurons, Brn-1 and Brn-2 appear to critically control the initiation of radial migration of cortical neurons. Brn-2 is thought to be involved in smooth muscle cell development and differentiation. Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. Brn-3a has two functional transactivating domains, one at the amino terminus and one at the carboxy terminus. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor.