POU3F3 antibody (AA 351-450)

Catalog No. ABIN1386623

Product Details anti-POU3F3 Antibody

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Binding Specificity: AA 351-450

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU3F3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Brain1/POU3F3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for POU3F3

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Alternative Name: Brain1/POU3F3 (POU3F3 Products)

Synonyms: BRN1 antibody, OTF8 antibody, Brn1 antibody, RHS1 antibody, Brn-1 antibody, Otf8 antibody, Skin1 antibody, brn1 antibody, nrl-20 antibody, nrl20-A antibody, pou1 antibody, pou23 antibody, wu:fb92g06 antibody, zfpou1 antibody, zp23 antibody, zp23pou antibody, BRN-1.1 antibody, ZP12POU antibody, brn1.1 antibody, pou12 antibody, wu:fc33a11 antibody, zp12 antibody, POU class 3 homeobox 3 antibody, POU domain, class 3, transcription factor 3 antibody, POU class 3 homeobox 3 S homeolog antibody, POU class 3 homeobox 3b antibody, POU class 3 homeobox 3a antibody, POU3F3 antibody, Pou3f3 antibody, pou3f3.S antibody, pou3f3b antibody, pou3f3a antibody

Background:

Synonyms: Brain 1, Brain specic homeobox/POU domain protein 1, Brain-1, Brain-specic homeobox/POU domain protein 1, Brain1, BRN 1, Brn-1, BRN1, class 3, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, OTF 8, OTF-8, OTF8, PO3 F3, PO3F 3, PO3F3, PO3F3_HUMAN, POU class 3 homeobox 3, POU domain, POU domain, class 3, transcription factor 3, POU3 F3, POU3F 3, Pou3f3, RHS 1, Rhs 2, RHS1, Rhs2, Skin 1, Skin1, transcription factor 3.

Background: The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU domain proteins. Expressed during the development of the forebrain and coexpressed in most layer II-V cortical neurons, Brn-1 and Brn-2 appear to critically control the initiation of radial migration of cortical neurons. Brn-2 is thought to be involved in smooth muscle cell development and differentiation. Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. Brn-3a has two functional transactivating domains, one at the amino terminus and one at the carboxy terminus. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor.