GNAS antibody (Internal Region)

Catalog No. ABIN571171

This Goat Polyclonal antibody specifically detects GNAS in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GNAS antibody (Internal Region) (ABIN571171)

Target: GNAS (GNAS Complex Locus (GNAS))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This GNAS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GNAS Antibody

Binding Specificity: Internal Region

Purpose: GNAS

Sequence: QAARSNSDGE KATK

Specificity: This antibody is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2. However, in Mouse it is expected to recognize reporterd isoforms GNASL (NP_963910.1) and XLas (NP_034439.2) only.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QAARSNSDGEKATK, from the internal region of the protein sequence according to NP_000507.1, NP_001070956.1, NP_001070957.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 45 kDa band observed in Human Cerebellum, Mouse and Rat Brain lysates, and in lysates of cell line KNRK (calculated MW of 45.6 kDa according to Human NP_963910.1, Mouse NP_963910.1 and Rat NP_062005.1). Recommended concentration: 0.1

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Alternative Name: GNAS

Background: GNAS, GNAS complex locus, AHO, C20orf45, GNAS1, GPSA, GSA, GSP, MGC33735, NESP, PHP1A, PHP1B, POH, dJ309F20.1.1, dJ806M20.3.3, OTTHUMP00000031742, OTTHUMP00000196026, OTTHUMP00000196030, adenylate cyclase-stimulating G alpha protein, guanine nucleotide bi

Gene ID: 2778, 14683, 24896

NCBI Accession: NP_000507, NP_001070956, NP_001070957

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis