RDH5 antibody (AA 19-31)

Catalog No. ABIN571175

The Goat Polyclonal anti-RDH5 antibody is suitable to detect RDH5 in samples from Human. It has been validated for ELISA, IHC, FACS and IF.

Quick Overview for RDH5 antibody (AA 19-31) (ABIN571175)

Target: RDH5 (Retinol Dehydrogenase 5 (11-Cis/9-Cis) (RDH5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RDH5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-RDH5 Antibody

Binding Specificity: AA 19-31

Purpose: RDH5 (aa 19-31)

Sequence: RDRQSLPASN AFV

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RDRQSLPASNAFV, from the internal region of the protein sequence according to NP_002896.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Retina shows staining of the Ganglion Cell Layer. Recommended concentration: 5-10 μg/mL.

Western Blot: Preliminary testing showed a band at approx..32 kDa in Human Heart and approx. 35 kDa in Human Breast lysate, after 0.3 μg/mL antibody staining. (calculated MW of 35.0 kDa according to NP_002896.2). Primary incubation 1 hour at room temperatur

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RDH5

Target: RDH5 (Retinol Dehydrogenase 5 (11-Cis/9-Cis) (RDH5))

Alternative Name: RDH5

Background: RDH5, retinol dehydrogenase 5 (11-cis/9-cis), FLJ39337, FLJ97089, HSD17B9, RDH1, SDR9C5, 11-cis RDH, 9-cis-retinol specific dehydrogenase, retinol dehydrogenase 1, retinol dehydrogenase 5 (11-cis and 9-cis), short chain dehydrogenase/reductase family 9C,

Gene ID: 5959, 19682

NCBI Accession: NP_002896