Dystroglycan antibody (Internal Region)

Catalog No. ABIN571179

This anti-Dystroglycan antibody is a Goat Polyclonal antibody detecting Dystroglycan in WB, ELISA, IHC, IF and FACS. Suitable for Human.

Quick Overview for Dystroglycan antibody (Internal Region) (ABIN571179)

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Dystroglycan antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Dystroglycan Antibody

Binding Specificity: Internal Region

Purpose: DAG1

Sequence: HVGKHEYFMH ATDK

Specificity: This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-HVGKHEYFMHATDK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4 kDa according to Human NP_004384.4 ). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Alternative Name: DAG1

Background: DAG1, dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1

Gene ID: 1605

NCBI Accession: NP_004384

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus