GLUT4 antibody (C-Term)

Catalog No. ABIN571189

This Goat Polyclonal antibody specifically detects GLUT4 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for GLUT4 antibody (C-Term) (ABIN571189)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GLUT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-GLUT4 Antibody

Binding Specificity: C-Term

Purpose: SLC2A4 / GLUT4

Sequence: TELEYLGPDE ND

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-TELEYLGPDEND

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40-45 kDa band observed in Mouse Heart lysates (calculated MW of 54.8 kDa according to Human NP_001033.1 and Mouse NP_033230.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fis

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Alternative Name: SLC2A4

Background: SLC2A4, solute carrier family 2 (facilitated glucose transporter), member 4, GLUT4, glucose transporter 4, insulin-responsive glucose transporter type 4

Gene ID: 6517, 20528, 25139

NCBI Accession: NP_001033

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effect