ETFB antibody (AA 231-243)

Catalog No. ABIN571193

The Goat Polyclonal anti-ETFB antibody has been validated for WB, IHC and ELISA. It is suitable to detect ETFB in samples from Human, Mouse and Rat.

Quick Overview for ETFB antibody (AA 231-243) (ABIN571193)

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ETFB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ETFB Antibody

Binding Specificity: AA 231-243

Purpose: ETFB (aa 231-243)

Sequence: PQRTAGVKVE TTE

Specificity: This antibody is expected to recognize both reported isoforms (NP_001976.1, NP_001014763.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-PQRTAGVKVETTE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 26 kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 27.8 kDa according to NP_001976.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ETFB

Target: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Alternative Name: ETFB

Background: ETFB, electron-transfer-flavoprotein, beta polypeptide, FP585, MADD, electron transfer flavoprotein beta subunit, electron transfer flavoprotein beta-subunit, electron transfer flavoprotein, beta polypeptide, electron-transferring-flavoprotein, beta polyp

Gene ID: 2109, 110826, 292845

NCBI Accession: NP_001976, NP_001014763