MGAT1 antibody (Internal Region)

Catalog No. ABIN571229

The Goat Polyclonal anti-MGAT1 antibody has been validated for WB and ELISA. It is suitable to detect MGAT1 in samples from Human.

Quick Overview for MGAT1 antibody (Internal Region) (ABIN571229)

Target: MGAT1 (Mannosyl (Alpha-1,3-)-Glycoprotein beta-1,2-N-Acetylglucosaminyltransferase (MGAT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MGAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-MGAT1 Antibody

Binding Specificity: Internal Region

Purpose: MGAT1

Sequence: QVEKVRTNDR

Specificity: Reported variants represent identical protein: NP_002397.2, NP_001108090.1, NP_001108091.1, NP_001108092.1, NP_001108089.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QVEKVRTNDR

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected HEK293 transiently expressing full-length Human MGAT1 (myc and DYKDDDDK tagged), a band of approx. 55 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibo

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MGAT1

Target: MGAT1 (Mannosyl (Alpha-1,3-)-Glycoprotein beta-1,2-N-Acetylglucosaminyltransferase (MGAT1))

Alternative Name: MGAT1

Background: MGAT1, mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, MGAT, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase, OTTHUMP00000161546

Gene ID: 4245, 17308, 81519

NCBI Accession: NP_002397