DCTN3 antibody (Internal Region)

Catalog No. ABIN571258

This Goat Polyclonal antibody specifically detects DCTN3 in ELISA, IHC, WB and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for DCTN3 antibody (Internal Region) (ABIN571258)

Target: DCTN3 (Dctn3) (Dynactin 3 (p22) (Dctn3))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This DCTN3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-DCTN3 Antibody

Binding Specificity: Internal Region

Purpose: DCTN3

Sequence: QVALGNISSK RE

Specificity: This antibody is expected to recognize both reported isoforms (NP_009165.1, NP_077324.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QVALGNISSKRE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 6-8 μg/mL.

Western Blot: Approx 20 kDa band observed in Human Liver lysates, and approx. 18 kDa in Rat Kidney lysates and in preliminary testing of Mouse Kidney lysate (calculated MW of 19.5 kDa according to Human NP_077324.1 and 21.1 kDa according to Rat NP_001102129.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DCTN3

Target: DCTN3 (Dctn3) (Dynactin 3 (p22) (Dctn3))

Alternative Name: DCTN3

Background: DCTN3, dynactin 3 (p22), DCTN-22, DCTN22, MGC111190, dynactin 3, dynactin 3, isoform 1, dynactin light chain

Gene ID: 11258, 53598, 362504

NCBI Accession: NP_009165, NP_077324

Pathways: M Phase