CYP24A1 antibody (Internal Region)

Catalog No. ABIN571265

The Goat Polyclonal anti-CYP24A1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect CYP24A1 in samples from Human and Rat.

Quick Overview for CYP24A1 antibody (Internal Region) (ABIN571265)

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CYP24A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CYP24A1 Antibody

Binding Specificity: Internal Region

Purpose: CYP24A1

Sequence: RKYDIQATDN

Specificity: This antibody is expected to recognize both reported isoforms (NP_000773.2, NP_001122387.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-RKYDIQATDN

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skin. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in Human and Rat Liver lysates (calculated MW of 51.2 kDa according to NP_001122387.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYP24A1

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Alternative Name: CYP24A1

Background: CYP24A1, cytochrome P450, family 24, subfamily A, polypeptide 1, CP24, CYP24, MGC126273, MGC126274, P450-CC24, 1,25-@dihydroxyvitamin D3 24-hydroxylase, 24-OHase, cytochrome P450 family 24 subfamily A polypeptide 1, cytochrome P450, subfamily XXIV (vitami

Gene ID: 1591

NCBI Accession: NP_000773, NP_001122387

Pathways: Metabolism of Steroid Hormones and Vitamin D