PCBP1 antibody (AA 223-234)

Catalog No. ABIN571267

This Goat Polyclonal antibody specifically detects PCBP1 in WB and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for PCBP1 antibody (AA 223-234) (ABIN571267)

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PCBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PCBP1 Antibody

Binding Specificity: AA 223-234

Purpose: PCBP1 (aa223-234)

Sequence: SIQGQHTISP LD

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-SIQGQHTISPLD

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in Human Peripheral Blood Nucleocytes lysates (calculated MW of 37.5 kDa according to NP_006187.2). The 38 kDa was also observed in Rat Spleen while a 37 kDa band was observed in Rat Thymus. Recommended concentratio

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PCBP1

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Alternative Name: PCBP1

Background: PCBP1, poly(rC) binding protein 1, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X, alpha-CP1, heterogeneous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein X, nucleic acid binding protein sub 2.3

Gene ID: 5093, 23983, 500242

NCBI Accession: NP_006187