NOX5 antibody (Internal Region)

Catalog No. ABIN571285

This Goat Polyclonal antibody specifically detects NOX5 in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for NOX5 antibody (Internal Region) (ABIN571285)

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-NOX5 Antibody

Binding Specificity: Internal Region

Purpose: NOX5

Sequence: EWHPFTISSA PEQKD

Specificity: This antibody is expected to recognize all reported isoforms (NP_078781.3, NP_001171708.1, NP_001171709.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-EWHPFTISSAPEQKD

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90 kDa band observed in Human Testis lysates (calculated MW of 86.4 kDa according to NP_078781.3). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NOX5

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Alternative Name: NOX5

Background: NOX5, NADPH oxidase, EF-hand calcium binding domain 5, MGC149776, MGC149777, NADPH oxidase 5, OTTHUMP00000164443, OTTHUMP00000164444

Gene ID: 79400

NCBI Accession: NP_078781, NP_001171708, NP_001171709

Pathways: Proton Transport